Cyprus-Forum.com

[Niki]

men have their good points, apart from the obvious.. who else would take the rubbish out ??

as long as there are batteries, men are unneccessary

There are electric jar lid openers?

[denizaksulu]

I'm pretty sure all he's offering is protein.

Did you do the "protein analysis" all by yourself, or was it done in the lab.!!??

Did someone mention Kjeldahl? I love it. Real chemistry.

Now that reminds me of the frustrating results with Biurets. Never the same answer twice ...

A distant blue memory ..... I bet it's all Kits now!

We leave the Biuret tests for the biochemistry classes and their food tests. Kid stuff.

In our department we stick to Colorimetric determination of Glutathione as a standard practical. Can get a bit tedious. The cost of fresh reagents spiralling.

We were talking Protein Assays, hence Biuret ... the other main one being Bradford's ... why are you talking about Glutathione?

And both Biuret and Bradfords are colorimetric... hence why I said blue.

Why do you need to determine Glutathione .... what are you working on? The Oxidase or Reductase?

The oxidase.

Are you talking of Barfoeds test? Your memory is failing you perhaps. Maybe Bradfords is a test I am unfamiliar with.

Nope ... My memory is not failing me even though it's been 10 years since I've done one ... ... really standards these days

Bradford Assay is a rapid and accurate method commonly used to determine the total protein concentration of a sample. The assay is based on the observation that the absorbance maximum for an acidic solution of Coomassie Brilliant Blue G-250 shifts from 465 nm to 595 nm when binding to protein occurs. Both hydrophobic and ionic interactions stabilize the anionic form of the dye, causing a visible color change. Within the linear range of the assay (~5-25 mcg/mL), the more protein present, the more Coomassie binds.

Reference
Bradford, M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. (1976) 72, 248-254.

Thanks for that info. Not a test we se at out teaching level. As I had pointed out the Berfoed and Buiret were the 'primitive' qualitative tests.

We do the Glutathione exp for teaching purposes only. Its not a Clinical (Path Lab). The objective is mainly teaching Colorimetry , calibration curves and other lab procedures.

I am trying to develope a method on our new HPLC, with limited success (so far). Having problems with the Buffered mobile phase and temperature control. I dont think I will be there long enough to finish the work. I will have to leave it to the young blood.

[Svetlana]

...erm...are you two going for the 'Most Off Topic Posts of the Year award'?


Lana

[denizaksulu]

...erm...are you two going for the 'Most Off Topic Posts of the Year award'?


Lana

OK, to make you happy I will begin a tirade......................


Its just the developing of this lovely blue colour. The colour of the Cypriot sky and the deep sea .Still off topic. I give up.

[Oracle]

...erm...are you two going for the 'Most Off Topic Posts of the Year award'?


Lana

Well it is a science slanted thread .... but to bring it back specifically to topic ...

Without men ....... we would probably carry out much more useful research instead of spending zillions of dollars to capture a quark or a boson ... and then just split them to blow people up!

(For pedants ... I know those are sub-atomic! I am pitching for the lay-man / woman.)

[denizaksulu]

...erm...are you two going for the 'Most Off Topic Posts of the Year award'?


Lana

Well it is a science slanted thread .... but to bring it back specifically to topic ...

Without men ....... we would probably carry out much more useful research instead of spending zillions of dollars to capture a quark or a boson ... and then just split them to blow people up!

(For pedants ... I know those are sub-atomic! I am pitching for the lay-man / woman.)

O, I think the forum has been too quiet lately. Lana is getting too bored with no warnings to issue. Seeing our 'peaceful' mini debate might render her 'useless'.

Apologies Lana.

Battle shall commence, if thats what you prefer.

Deepest regards


Now back to Glu................................

[perdike]

...erm...are you two going for the 'Most Off Topic Posts of the Year award'?


Lana

Well it is a science slanted thread .... but to bring it back specifically to topic ...

Without men ....... we would probably carry out much more useful research instead of spending zillions of dollars to capture a quark or a boson ... and then just split them to blow people up!

(For pedants ... I know those are sub-atomic! I am pitching for the lay-man / woman.)

O, I think the forum has been too quiet lately. Lana is getting too bored with no warnings to issue. Seeing our 'peaceful' mini debate might render her 'useless'.

Apologies Lana.

Battle shall commence, if thats what you prefer.

Deepest regards


Now back to Glu................................

Glucosamine . . said to be good for bodybuilding males, so they can take the rubbish out!

[Oracle]

Glu ...... is the standard abbreviation for the amino acid Glutamate (Glutamic acid).

[humanist]

Oracles my dear a world without men would be absolutely boring

[Oracle]

Oracles my dear a world without men would be absolutely boring

I know my darling ... dread the day ... fortunately, I am unlikely to be around, should this come to pass.

One must identify putative problems before they arise, in order to set into motion, research to alleviate.

The article (link on OP), suggests ways we can salvage the much needed sexy "Y" chromosome .....