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[denizaksulu]

I'm only sorry I won't be around to see that puny tiny little Y chromosome wilt and drop off.

Who needs them?

Well you only say that because you are fortunate to have two "X" chromosomes ..... but what of those poor males battling to hold on to their diminishing, shrivelling genes?

All I'm saying is that from my limited (what a loser HE was!) experience I have noticed that the y thingy is becoming more and more icky.

I heard rumours that you are pregnant and expecting your first child Tania. Indeed that you are entering your third trimester.

Are they true?

(Teenage pregnancies )

Big deal And anyway its not like that at all. I know who the father is and he doesn't even want a dna test cause he's pretty much sure its him as well.

That sounds iffy. To say pretty sure, means that there was doubt. Were you two-timing him? Naughty girl. Lets hope it is him.

[denizaksulu]

I'm pretty sure all he's offering is protein.

Did you do the "protein analysis" all by yourself, or was it done in the lab.!!??

Did someone mention Kjeldahl? I love it. Real chemistry.

[Oracle]

I'm pretty sure all he's offering is protein.

Did you do the "protein analysis" all by yourself, or was it done in the lab.!!??

Did someone mention Kjeldahl? I love it. Real chemistry.

Now that reminds me of the frustrating results with Biurets. Never the same answer twice ...

A distant blue memory ..... I bet it's all Kits now!

[denizaksulu]

I'm pretty sure all he's offering is protein.

Did you do the "protein analysis" all by yourself, or was it done in the lab.!!??

Did someone mention Kjeldahl? I love it. Real chemistry.

Now that reminds me of the frustrating results with Biurets. Never the same answer twice ...

A distant blue memory ..... I bet it's all Kits now!

We leave the Biuret tests for the biochemistry classes and their food tests. Kid stuff.

In our department we stick to Colorimetric determination of Glutathione as a standard practical. Can get a bit tedious. The cost of fresh reagents spiralling.

[Oracle]

I'm pretty sure all he's offering is protein.

Did you do the "protein analysis" all by yourself, or was it done in the lab.!!??

Did someone mention Kjeldahl? I love it. Real chemistry.

Now that reminds me of the frustrating results with Biurets. Never the same answer twice ...

A distant blue memory ..... I bet it's all Kits now!

We leave the Biuret tests for the biochemistry classes and their food tests. Kid stuff.

In our department we stick to Colorimetric determination of Glutathione as a standard practical. Can get a bit tedious. The cost of fresh reagents spiralling.

We were talking Protein Assays, hence Biuret ... the other main one being Bradford's ... why are you talking about Glutathione?

And both Biuret and Bradfords are colorimetric... hence why I said blue.

Why do you need to determine Glutathione .... what are you working on? The Oxidase or Reductase?

[GorillaGal]

Dated via the internet ? and you have met these people ? Excuse my ignorance (rudeness), but are you not letting yourself in for trouble.

here here! sprry my timing is way off from the ret of you, but let me say this: i have met alot of men via the internet, including the GC i am so madly in love with, and it iw an excellent way to meet me. i have never really had a bad experience, and have met several great guys whom, although we may not have made a love connection, have remained my friends. it's far better than meeting a guy in a bar.

[GorillaGal]

men have their good points, apart from the obvious.. who else would take the rubbish out ??

as long as there are batteries, men are unneccessary

[denizaksulu]

I'm pretty sure all he's offering is protein.

Did you do the "protein analysis" all by yourself, or was it done in the lab.!!??

Did someone mention Kjeldahl? I love it. Real chemistry.

Now that reminds me of the frustrating results with Biurets. Never the same answer twice ...

A distant blue memory ..... I bet it's all Kits now!

We leave the Biuret tests for the biochemistry classes and their food tests. Kid stuff.

In our department we stick to Colorimetric determination of Glutathione as a standard practical. Can get a bit tedious. The cost of fresh reagents spiralling.

We were talking Protein Assays, hence Biuret ... the other main one being Bradford's ... why are you talking about Glutathione?

And both Biuret and Bradfords are colorimetric... hence why I said blue.

Why do you need to determine Glutathione .... what are you working on? The Oxidase or Reductase?

The oxidase.

Are you talking of Barfoeds test? Your memory is failing you perhaps. Maybe Bradfords is a test I am unfamiliar with.

[Oracle]

I'm pretty sure all he's offering is protein.

Did you do the "protein analysis" all by yourself, or was it done in the lab.!!??

Did someone mention Kjeldahl? I love it. Real chemistry.

Now that reminds me of the frustrating results with Biurets. Never the same answer twice ...

A distant blue memory ..... I bet it's all Kits now!

We leave the Biuret tests for the biochemistry classes and their food tests. Kid stuff.

In our department we stick to Colorimetric determination of Glutathione as a standard practical. Can get a bit tedious. The cost of fresh reagents spiralling.

We were talking Protein Assays, hence Biuret ... the other main one being Bradford's ... why are you talking about Glutathione?

And both Biuret and Bradfords are colorimetric... hence why I said blue.

Why do you need to determine Glutathione .... what are you working on? The Oxidase or Reductase?

The oxidase.

Are you talking of Barfoeds test? Your memory is failing you perhaps. Maybe Bradfords is a test I am unfamiliar with.

Nope ... My memory is not failing me even though it's been 10 years since I've done one ... ... really standards these days

Bradford Assay is a rapid and accurate method commonly used to determine the total protein concentration of a sample. The assay is based on the observation that the absorbance maximum for an acidic solution of Coomassie Brilliant Blue G-250 shifts from 465 nm to 595 nm when binding to protein occurs. Both hydrophobic and ionic interactions stabilize the anionic form of the dye, causing a visible color change. Within the linear range of the assay (~5-25 mcg/mL), the more protein present, the more Coomassie binds.

Reference
Bradford, M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. (1976) 72, 248-254.

[Filitsa]

Men are necessary evils like automobiles, cell phones, computers, and handbags, among other things.